Biomarkers of pollution in soils irrigated with wastewater in Tunisia
Identifieur interne : 000430 ( Main/Exploration ); précédent : 000429; suivant : 000431Biomarkers of pollution in soils irrigated with wastewater in Tunisia
Auteurs : F. Ben Fredj [Japon] ; M. Irie [Japon] ; J. Han [Japon] ; A. Charef [Tunisie] ; A. Ghrabi [Tunisie] ; H. Isoda [Japon]Source :
- Transactions on ecology and the environment [ 1746-448X ] ; 2010.
Descripteurs français
- Pascal (Inist)
- Marqueur biologique, Pollution sol, Eau usée, Réutilisation déchet, Irrigation, Elément trace, Toxique, Végétation, Eau souterraine, Santé publique, In vitro, Essai biologique, Métal lourd, Nickel, Cadmium, Plomb, Métal zérovalent, Fer, Station épuration, Profil profondeur, Distribution concentration, Activité biologique, Oestrogène, Identification, Cytotoxicité, Tunisie, Bassin méditerranéen, Valorisation, Gestion déchet, Santé et environnement, Hormone stéroïde sexuelle, Toxicité.
- Wicri :
- geographic : Tunisie.
- topic : Eau usée, Irrigation, Eau souterraine, Santé publique, Métal lourd, Nickel, Cadmium, Plomb, Fer.
English descriptors
- KwdEn :
- Bioassay, Biological activity, Biological marker, Cadmium, Concentration distribution, Cytotoxicity, Depth profile, Estrogen, Ground water, Health and environment, Heavy metal, Identification, In vitro, Iron, Irrigation, Lead, Mediterranean Basin, Nickel, Poison, Public health, Sewage treatment plant, Sex steroid hormone, Soil pollution, Toxicity, Trace element, Tunisia, Upgrading, Vegetation, Waste management, Waste reuse, Waste water, Zerovalent metal.
Abstract
Wastewater reuse for irrigation is expected in the Mediterranean basin as a sustainable alternative with socio-economic benefits. However, wastewater carries considerable amounts of harmful chemicals and trace toxic metals that are able to accumulate in soil. This pollution loading is threatening both the vegetation and the groundwater and, consequently, human health. Therefore, in this study, in vitro bioassays were used to evaluate the adverse effects of wastewater and soil extract samples, on mammalian cells, with respect to heavy metal content (Ni, Cd, Pb, Fe and Al). The irrigation water, from a wastewater treatment plant, was sampled at Zaouit-Sousse perimeter in Tunisia. A representative soil profile irrigated with wastewater (0-90 cm) and a non-irrigated profile (0-40 cm), serving as a control, were sampled in the olive-tree-occupied perimeter. The estrogenic activity of the samples was investigated using the E-screen assay, while the heat shock protein (HSP) 47 assay aimed to determine the stress response of HSP 47-promoter-transfected cells following sample addition. Furthermore, proteomics analyses were performed, aiming to establish specific biomarkers of environmental pollutants. The irrigation water samples induced a high estrogenic activity when the MCF-7 cells were treated at 10% due to the presence of estrogenic compounds. The E-screen assay revealed a cytotoxic effect of the surface soil samples (0-20 cm), even at low concentrations (0.001%). Furthermore, the proteomics experiments allowed the identification of specific biomarkers for estrogenicity and cytotoxicity on MCF-7 cells. Moreover, significant stress response was observed following the incubations of the HSP 47-promoter-transfected cells with wastewater samples, even at 0.1%, and soil extracts (0-60 cm) at concentrations as low as 1%. Interestingly, no stress response or cytotoxic effect was observed for the control soil or for deep layers below 60 cm. These biological techniques could be extremely useful to evaluate the impact of wastewater reuse.
Affiliations:
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Ghrabi</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Water Researches and Technologies Center, Technopark of Borj-Cedria</s1><s3>TUN</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Tunisie</country><wicri:noRegion>Water Researches and Technologies Center, Technopark of Borj-Cedria</wicri:noRegion></affiliation></author><author><name sortKey="Isoda, H" sort="Isoda, H" uniqKey="Isoda H" first="H." last="Isoda">H. Isoda</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Graduate School of Life and Environmental Sciences, University of Tsukuba</s1><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Graduate School of Life and Environmental Sciences, University of Tsukuba</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Transactions on ecology and the environment</title><idno type="ISSN">1746-448X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Transactions on ecology and the environment</title><idno type="ISSN">1746-448X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bioassay</term><term>Biological activity</term><term>Biological marker</term><term>Cadmium</term><term>Concentration distribution</term><term>Cytotoxicity</term><term>Depth profile</term><term>Estrogen</term><term>Ground water</term><term>Health and environment</term><term>Heavy metal</term><term>Identification</term><term>In vitro</term><term>Iron</term><term>Irrigation</term><term>Lead</term><term>Mediterranean Basin</term><term>Nickel</term><term>Poison</term><term>Public health</term><term>Sewage treatment plant</term><term>Sex steroid hormone</term><term>Soil pollution</term><term>Toxicity</term><term>Trace element</term><term>Tunisia</term><term>Upgrading</term><term>Vegetation</term><term>Waste management</term><term>Waste reuse</term><term>Waste water</term><term>Zerovalent metal</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Marqueur biologique</term><term>Pollution sol</term><term>Eau usée</term><term>Réutilisation déchet</term><term>Irrigation</term><term>Elément trace</term><term>Toxique</term><term>Végétation</term><term>Eau souterraine</term><term>Santé publique</term><term>In vitro</term><term>Essai biologique</term><term>Métal lourd</term><term>Nickel</term><term>Cadmium</term><term>Plomb</term><term>Métal zérovalent</term><term>Fer</term><term>Station épuration</term><term>Profil profondeur</term><term>Distribution concentration</term><term>Activité biologique</term><term>Oestrogène</term><term>Identification</term><term>Cytotoxicité</term><term>Tunisie</term><term>Bassin méditerranéen</term><term>Valorisation</term><term>Gestion déchet</term><term>Santé et environnement</term><term>Hormone stéroïde sexuelle</term><term>Toxicité</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Tunisie</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Eau usée</term><term>Irrigation</term><term>Eau souterraine</term><term>Santé publique</term><term>Métal lourd</term><term>Nickel</term><term>Cadmium</term><term>Plomb</term><term>Fer</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wastewater reuse for irrigation is expected in the Mediterranean basin as a sustainable alternative with socio-economic benefits. However, wastewater carries considerable amounts of harmful chemicals and trace toxic metals that are able to accumulate in soil. This pollution loading is threatening both the vegetation and the groundwater and, consequently, human health. Therefore, in this study, in vitro bioassays were used to evaluate the adverse effects of wastewater and soil extract samples, on mammalian cells, with respect to heavy metal content (Ni, Cd, Pb, Fe and Al). The irrigation water, from a wastewater treatment plant, was sampled at Zaouit-Sousse perimeter in Tunisia. A representative soil profile irrigated with wastewater (0-90 cm) and a non-irrigated profile (0-40 cm), serving as a control, were sampled in the olive-tree-occupied perimeter. The estrogenic activity of the samples was investigated using the E-screen assay, while the heat shock protein (HSP) 47 assay aimed to determine the stress response of HSP 47-promoter-transfected cells following sample addition. Furthermore, proteomics analyses were performed, aiming to establish specific biomarkers of environmental pollutants. The irrigation water samples induced a high estrogenic activity when the MCF-7 cells were treated at 10% due to the presence of estrogenic compounds. The E-screen assay revealed a cytotoxic effect of the surface soil samples (0-20 cm), even at low concentrations (0.001%). Furthermore, the proteomics experiments allowed the identification of specific biomarkers for estrogenicity and cytotoxicity on MCF-7 cells. Moreover, significant stress response was observed following the incubations of the HSP 47-promoter-transfected cells with wastewater samples, even at 0.1%, and soil extracts (0-60 cm) at concentrations as low as 1%. Interestingly, no stress response or cytotoxic effect was observed for the control soil or for deep layers below 60 cm. These biological techniques could be extremely useful to evaluate the impact of wastewater reuse.</div></front></TEI><affiliations><list><country><li>Japon</li><li>Tunisie</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Ben Fredj, F" sort="Ben Fredj, F" uniqKey="Ben Fredj F" first="F." last="Ben Fredj">F. Ben Fredj</name></noRegion><name sortKey="Han, J" sort="Han, J" uniqKey="Han J" first="J." last="Han">J. Han</name><name sortKey="Irie, M" sort="Irie, M" uniqKey="Irie M" first="M." last="Irie">M. Irie</name><name sortKey="Isoda, H" sort="Isoda, H" uniqKey="Isoda H" first="H." last="Isoda">H. Isoda</name></country><country name="Tunisie"><noRegion><name sortKey="Charef, A" sort="Charef, A" uniqKey="Charef A" first="A." last="Charef">A. Charef</name></noRegion><name sortKey="Ghrabi, A" sort="Ghrabi, A" uniqKey="Ghrabi A" first="A." last="Ghrabi">A. Ghrabi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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